Clinical Detection of Peripheral Blood Natural Killer Cell Activity
نویسندگان
چکیده
منابع مشابه
Epstein-Barr virus infection of human natural killer cell lines and peripheral blood natural killer cells.
Although considerable part of natural killer (NK) cell neoplasms possess EBV genome, there has been no direct evidence that EBV infects human NK cells in vitro. In this study, we demonstrated EBV entry into NK cells using a recombinant EBV, which contains enhanced green fluorescent protein (EGFP) gene in its genome (EGFP-EBV). After 48 h of exposure to EGFP-EBV, we detected EGFP signals in appr...
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We have developed a simple assay for the detection of peripheral blood natural killer cells (NK) metabolic activity based on tetrazolium reduction reaction after the cultivation of isolated NK cells with sperm cells. We have adapted a reliable, inexpensive and easy to prepare method in conjunction with the EZ4U system, while target NK cells' isolation was solved using Dynabeads immunomagnetic t...
متن کاملNatural killer (NK) activity of normal human peripheral blood lymphocytes against erythroleukemic cell line K 562.
Human peripheral blood lymphocytes of healthy donors showed significant levels of natural killer (NK) activity against erythroleukemic cell line K 562 in 3-hour 51Cr release assays. The levels of NK activity showed much individual variation, which was considered to be an individual trait that varied little under physiological conditions. The NK activity of mononuclear leukocytes from cord blood...
متن کاملSuppression of peripheral blood natural killer cell activity by excess thyroid hormone.
Natural killer (NK) cells were assessed in patients with hyperthyroxinemia due to Graves' disease or treatment with thyroxine (T4). Cytolytic activity was measured with 51Cr-labeled K562 tumor cells and NK enumeration was by flow cytometry using NKH-1 monoclonal antibody to identify the relevant surface marker. Activity was uniformly decreased in association with hyperthyroxinemia, regardless o...
متن کاملNatural killer cells in human peripheral blood and primary cutaneous natural killer cell lymphomas may express cutaneous lymphocyte antigen.
In normal human peripheral blood, cutaneous lymphocyte antigen is expressed by memory T cells, suggesting a specific tissue-homing population of T cells. In this study it is demonstrated that 6% of CD56 + natural killer cells in peripheral blood also express cutaneous lymphocyte antigen (CLA). It was also detected that most of the tumor cells in primary cutaneous nasal-type natural killer cell ...
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ژورنال
عنوان ژورنال: Journal of Biosciences and Medicines
سال: 2021
ISSN: 2327-5081,2327-509X
DOI: 10.4236/jbm.2021.95004